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KMID : 0903619700070010095
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Journal of the Korean Society for Horticultural Science
1970 Volume.7 No. 1 p.95 ~ p.98
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Fluorescent Staining for Pollen Tubes of Certain Ornamental Plants
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Abstract
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In vitro and in situ growth of Crinum asiaticum, Petunia inflate, and Galphimia glauca pollen tubes were tested and observed for fluorescent staining with tetracycline and other substances with reference to the anilin-blue fluorescence microscopy.
Tetracycline was found to demonstrate as unique fluorescence on both in vitro and in situ pollen tubes as anion blue.
Observations with tetracycline revealed that hourly pollen-tube growth in vitro proceeded at much slower rate than in situ in all ornamental species examined, and in vitro growth slows in down much earlier period of time than in situ. Thus pollen tubes of the longer style species seemed to grow faster in both growth conditions than the shorter ones.
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